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Your Position: Home > Protein > IL-1 RII > IL2-H4256

Human IL-1 RII / CD121b Protein, Fc Tag

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  • Synonym
    CD121b,IL1RB,IL1R2,CDw121b
  • Source
    Human IL-1 RII, Fc Tag(IL2-H4256) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1).
    Predicted N-terminus: Phe 14
  • Molecular Characterization
    IL-1 RII Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 63.9 kDa. The protein migrates as 70-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 RII SDS-PAGE

Human IL-1 RII, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-1 RII ELISA

Immobilized Human IL-1 beta, premium grade (Cat. No. ILB-H4110) at 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) with a linear range of 8-63 ng/mL (QC tested).

Bioactivity-SPR
 IL-1 RII SPR

Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. ILB-H4110) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

 IL-1 RII SPR

Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-1 beta, His Tag (Cat. No. ILB-H51H3) with an affinity constant of 0.571 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Interleukin-1 receptor type 2 (IL1R2) is also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
  • Clinical and Translational Updates

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