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Your Position: Home > All Other Proteins > IL-1 RII > Human IL1R2 CD121b Protein, With C-Fc Tag

Human IL-1 RII / CD121b Protein, Fc Tag

  • Synonym
    CD121b,IL1RB,IL1R2,CDw121b
  • Source
    Human IL-1 RII, Fc Tag (IL2-H4256) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1).
    Predicted N-terminus: Phe 14
  • Molecular Characterization
    IL-1 RII(Phe 14 - Glu 343) NP_004624.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 64 kDa. The protein migrates as 68-74 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) SDS-PAGE gel

Human IL-1 RII, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human IL-1 RII, Fc TagHuman IL-1 RII, Fc Tag (Cat. No. IL2-H4256) ELISA bioactivity

Immobilized Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) at 10 μg/mL (100 µL/well),can bind Biotinylated Human IL-1 alpha (Cat. No. ILA-H8213) with a linear range of 0.05-1 μg/mL (QC tested).

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