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Your Position: Home > Membrane Protein > EDNRB > EDB-H52P7

Human EDNRB Full Length Protein-VLP (HEK293)

  • Synonym
    EDNRB,endothelin receptor type B,ABCDS, ET-B, ET-BR, ETB, ETB1, ETBR, ETRB, HSCR, HSCR2, WS4A
  • Source
    Human EDNRB Full Length Protein-VLP(EDB-H52P7) is expressed from human 293 cells (HEK293). It contains AA Glu 27 - Ser 442 (Accession # P24530-1 ).
    Predicted N-terminus: Asp
  • Molecular Characterization

    Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >80% as determined by SDS-PAGE.

  • Formulation

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

    Supplied as 0.2 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 EDNRB ELISA

Immobilized Human EDNRB Full Length Protein-VLP (Cat. No. EDB-H52P7) at 5 μg/mL (100 μL/well) can bind Anti-EDNRB Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Identity-DLS
 EDNRB DLS

The mean peak Radius of VLP is 65-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

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