>Immune Checkpoint Proteins
>Human SIRP alpha / CD172a Protein, mouse IgG1 Fc Tag (HPLC-verified)
This protein carries a mouse IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) was greater than 90% as determined by SEC-HPLC.
FACS assay shows that recombinant Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml (Routinely tested).
FACS analysis shows that the binding of Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/ml. IC50=0.1318 μg/ml (Routinely tested).
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Price(USD) : $280.00
Price(USD) : $1980.00
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