>Human Interferon gamma /IFNG Protein,HEK293 expressed,ActiveMax
This protein carries no "tag".
The protein has a calculated MW of 16.8 kDa. The protein migrates as 17 kDa,20 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human IFN-gamma on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The bio-activity of IFN-gamma（Cat. No. IFG-H4211）was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL (Routinely tested).
Authors: A.E. Ross, et al.
Journal: ANAL CHIM ACTA 2017
Authors: Razaghi A, et al.
Journal: Exp Cell Res 2017
Application: Cell Culture
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