|RAS135-C01||Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate||1 plate|
|RAS135-C02||Glycoprotein E (VZV) Standard||30 μg|
|RAS135-C03||HRP-Anti-Glycoprotein E (VZV) Antibody||15 μg|
|RAS135-C04||10xWashing Buffer||50 mL|
|RAS135-C05||2xDilution Buffer||50 mL|
|RAS135-C06||Substrate Solution||12 mL|
|RAS135-C07||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Add your sample to the plate, take the Glycoprotein E (VZV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted HRP-Anti-Glycoprotein E (VZV) Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using HRP-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 3.8 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $580.00
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