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Your Position: Home > > Glycoprotein E (VZV) > RAS-A135

Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit (For Vaccine Development)

For research use only.
IDComponentsSize
RAS135-C01Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate1 plate
RAS135-C02Glycoprotein E (VZV) Standard30 μg
RAS135-C03HRP-Anti-Glycoprotein E (VZV) Antibody15 μg
RAS135-C0410xWashing Buffer 50 mL
RAS135-C052xDilution Buffer50 mL
RAS135-C06Substrate Solution12 mL
RAS135-C07Stop Solution7 mL
  • Background
    Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,Its helpful to develop the Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit to quantitative detection the VZV gE antigen in vaccine samples during the manufacture and quality control of vaccine development.
  • Application
    This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein E (VZV gE) in vaccine samples.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Glycoprotein E (VZV). The kit consists of Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate and Glycoprotein E (VZV) Standard and HRP-Anti-Glycoprotein E (VZV) Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the Glycoprotein E (VZV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-Anti-Glycoprotein E (VZV) Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for The assay protocol.
 Glycoprotein E (VZV) ELISA

Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using HRP-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 3.8 ng/mL (QC tested).

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