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Your Position: Home > Non SARS-Cov-2 Kits > IgG > AHG-Y69

Anti-Human IgG-Acridine ester (MALS verified)

  • Source
    Anti-Human IgG-Acridine ester antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IgG.
  • Isotype
    Mouse IgG1 | Mouse Kappa
  • Specificity
    This product is a specific antibody specifically reacts with IgG.
  • Application
    MPCLIA
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH6.3.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG SDS-PAGE

Anti-Human IgG-Acridine ester on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IgG MALS images

The purity of Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) is more than 90% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 IgG MPCLIA

Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 IgG MPCLIA

The MPCLIA assay shows that Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) is stable at 37°C for 48 hours.

 IgG MPCLIA

The MPCLIA assay shows that Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) is stable after freezing and thawing 3 times.

  • Background
    Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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