Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > LIGHT > EP-148

LIGHT : HVEM[Biotinylated] Inhibitor Screening ELISA Kit

For research use only.

Order Now

EP148-C01High-bind Plate1 plate
EP148-C02Human LIGHT15 μg
EP148-C03Biotinylated Human HVEM10 μg
EP148-C04Anti-LIGHT Neutralizing Antibody20 μg
EP148-C05Streptavidin-HRP10 μg
EP148-C06Coating Buffer12 mL
EP148-C0710xWashing Buffer 50 mL
EP148-C08Blocking Buffer50 mL
EP148-C09Substrate Solution12 mL
EP148-C10Stop Solution7 mL
  • Background
    Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
  • Application
    This kit is developed for screening for inhibitors of human LIGHT binding to human HVEM.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new LIGHT pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human HVEM to immobilized human LIGHT in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated HVEM protein, a human LIGHT protein, an anti-LIGHT neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human LIGHT.

    b) Add your molecule of interest to the tests.

    c) Add human HVEM-Biotin to bind the coated LIGHT.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to LIGHT: HVEM binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to DS document for The assay protocol.

Serial dilutions of Anti- LIGHT Neutralizing antibody (Catalog # EP148-C04) (1:1 serial dilution, from 5μg/mL to 0.002μg/mL) was added into LIGHT: HVEM[Biotinylated] binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Comments (0)

ETD of in-stock products: 2 business days

Price(USD) : $650.00

Promotion & Exhibitions

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message