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Your Position: Home > Non SARS-Cov-2 Kits > LIGHT > EP-148

LIGHT : HVEM[Biotinylated] Inhibitor Screening ELISA Kit

For research use only.
IDComponentsSize
EP148-C01High-bind Plate1 plate
EP148-C02Human LIGHT15 μg
EP148-C03Biotinylated Human HVEM10 μg
EP148-C04Anti-LIGHT Neutralizing Antibody20 μg
EP148-C05Streptavidin-HRP10 μg
EP148-C06Coating Buffer12 mL
EP148-C0710xWashing Buffer 50 mL
EP148-C08Blocking Buffer50 mL
EP148-C09Substrate Solution12 mL
EP148-C10Stop Solution7 mL
  • Background
    Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
  • Application
    This kit is developed for screening for inhibitors of human LIGHT binding to human HVEM.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new LIGHT pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human HVEM to immobilized human LIGHT in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated HVEM protein, a human LIGHT protein, an anti-LIGHT neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human LIGHT.

    b) Add your molecule of interest to the tests.

    c) Add human HVEM-Biotin to bind the coated LIGHT.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to LIGHT: HVEM binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to DS document for The assay protocol.
 LIGHT ELISA

INHIBITION OF LIGHT: HVEM[Biotinylated] BINDING BY ANTI-LIGHT NEUTRALIZING ANTIBODY
Serial dilutions of Anti- LIGHT Neutralizing antibody (Catalog # EP148-C04) (1:1 serial dilution, from 5μg/mL to 0.002μg/mL) was added into LIGHT: HVEM[Biotinylated] binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $650.00

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  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

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