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| ID | Components | Size |
| EP152-C01 | High-bind Plate | 1 plate |
| EP152-C02 | Human SIRP alphaV2 | 20 μg |
| EP152-C03 | Anti-CD47 Neutralizing Antibody | 20 μg |
| EP152-C04 | Human CD47-Biotin | 10 μg |
| EP152-C05 | Streptavidin-HRP | 10 μg |
| EP152-C06 | Coating Buffer | 12 mL |
| EP152-C07 | 10xWashing Buffer | 50 mL |
| EP152-C08 | Blocking Buffer | 50 mL |
| EP152-C09 | Substrate Solution | 12 mL |
| EP152-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human CD47 binding to human SIRP alphaV2.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human SIRP alphaV2.
b) Add your molecule of interest to the tests.
c) Add human CD47-Biotin to bind the coated human SIRP alphaV2.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit CD47 [Biotinylated] : SIRP alphaV2 Rbinding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF CD47 [BIOTINYLATED]: SIRP ALPHAV2 BINDING BY ANTI-CD47 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD47 Neutralizing Antibody (Catalog # EP152-C03) (1:1 serial dilution, from 10 μg/mL to 0.078 μg/mL (68.493-0.535 nM)) was added into SIRP alphaV2: CD47-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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