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Your Position: Home > MabAffinity Chromatography Media > MabAffinity rProtein A Prepacked Column

MabAffinity rProtein A Prepacked Column

MabAffinity rProtein A Agarose High Flow Resin is a classic protein A affinity medium for fast and efficient purification of monoclonal and polyclonal antibodies at any scale. The high specificity of rProtein A ligand to Fc domain of IgGs gives a high purification factor in a single chromatography step. Superior coupling chemistry ensures both high capacity and low leakage.
Features
  1. Classic rProtein A resin suitable for different applications, including purification of poly or monoclonal antibodies of different species and subclasses and isolation of immune complex

  2. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification
CompositionHighly cross-linked Agarose
Average particle size90 micron
Form5ml prepacked column;
LigandrProtein A
Coupling chemistryEpoxy
Dynamic binding capacity1> 150mg IgG /5ml prepacked column
Recommended flow rate1-5ml/min for 5ml prepacked column
Column dimension1.6x2.5 cm for 5ml prepacked column
Maximum Pressure Drop0.3Mpa
Chemical stabilityStable in all aqueous buffers commonly used in protein A chromatography
pH working range3-10
pH CIP range (short term)2-11
CIP stability8M Urea, 6M Gua-HCl or 10mM NaOH+1M NaCl, 0.1M acetic acid
Temperature stability22-40oC
Storage20% Ethanol
Shelf life5 years
  1. Determined at 10% breakthrough at 4min residence time

  2. Delivered at room temperature, and recommended long-term storage at 2-8 oC

MabAffinity rProtein A Agarose High Flow Resin belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity rProtein A Agarose High Flow are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

Related Products
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MA-0422-C5MabAffinity rProtein A Prepacked Column 5ml5ml ($290)
MA-0422-02MabAffinity rProtein A Agarose High Flow25ml ($635)
MA-0422-03MabAffinity rProtein A Agarose High Flow100ml ($1200)
MG-0422-C5MabAffinity Protein G Prepacked Column 5ml5ml ($350)
MG-0422-02MabAffinity Protein G Agarose High Flow25ml ($900)
MG-0422-03MabAffinity Protein G Agarose High Flow100ml ($1500)
ML-0422-C5MabAffinity Protein L Prepacked Column 5ml5ml ($395)
ML-0422-01MabAffinity Protein L Agarose High Flow5ml ($390)
ML-0422-02MabAffinity Protein L Agarose High Flow25ml ($1400)
ML-0422-03MabAffinity Protein L Agarose High Flow100ml ($2900)
FK-0422-C1FabAffinity KBP Prepacked Column 1ml1ml ($220)
FK-0422-C5FabAffinity KBP Prepacked Column 5ml5ml ($650)
FK-0422-01FabAffinity KBP Agarose High Flow5ml ($550)

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Order Details

Price(USD) : $290.00

Inventory : In stock

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