Login Register
icon_bulk_orderBulk order Acrobiosystems for English
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > IL-1 beta > CRS-B002

ClinMax™ Human IL-1β ELISA Kit

For research use only.
Product Details
Assay TypeSandwich-ELISA
Regulatory StatusRUO
Sensitivity<1.0 pg/mL
Standard Curve Range4.69 pg/mL-600 pg/mL
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of human IL-1β in serum, plasma and cell supernatant.
Sample volume50 μL
Materials Provided
CRB002-C01Pre-coated Anti-IL-1β Antibody Microplate1 plate
CRB002-C02Human IL-1β Standard20 μg×2
CRB002-C03Biotin-Anti-IL-1β Antibody Con. Solution100 μL
CRB002-C04Biotin-Antibody Dilution Buffer8 mL
CRB002-C05Streptavidin-HRP Con. Solution500 μL
CRB002-C06Streptavidin-HRP Dilution Buffer15 mL
CRB002-C0720× Washing Buffer50 mL
CRB002-C081× Dilution Buffer15 mL×2
CRB002-C09Substrate Solution12 mL
CRB002-C10Stop Solution6 mL
  • Background
    Human Interleukin-1beta (IL-1β) OneStep ELISA Kit is a one-step quick immunoassay designed to measure human IL-1beta levels in cell culture supernates, serum, and plasma. This assay kit is a standard sandwich-ELISA format. It contains recombinant human IL-1beta and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human IL-1beta concentrations. The specificity has been verified.
  • Application

    The kit is developed for quantitative detection of IL-1beta in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the concentrations of human Interleukin 2 (IL-1β) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-1β Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-1β Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B002 is less than 1.0 pg/mL.

  • Background: IL-1 beta
    Interleukin-1β, IL-1β
    IL-1β is a potent pro-inflammatory cytokine. IL-1β is produced and secreted by various cell types, such as immune cells, fibroblasts, or cancer cells. The IL-1β gene is induced after "priming" of the cells and a second signal is required to allow IL-1β maturation by inflammasome-activated caspase-1. IL-1β is then released and leads to transcription of target genes through its ligation with IL-1R1 on target cells. IL-1β expression and maturation are guided by gene polymorphisms and by the cellular context. In cancer, IL-1β has pleiotropic effects on immune cells, angiogenesis, cancer cell proliferation, migration, and metastasis.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

ETD of in-stock products: 2 business days

Price(USD) : $498.00

Price(USD) :

Promotion & Exhibitions

Drug Development Status

  • Number of Launched Drugs:5 Details
  • Number of Drugs in Clinical Trials:10 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message