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MabAffinity Protein G Prepacked Column

MabAffinity Protein G Agarose High Flow Resin, with rProtein G coupled to high flow Agarose matrix, is a very popular affinity resin for poly and monoclonal antibody/fragments purification and isolation of immune complex. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Compared to protein A, protein G binds more strongly to polyclonal IgG from cow, sheep and horse. Furthermore, unlike protein A, MabAffinity Protein G Agarose High Flow Resin can be used successfully to capture polyclonal rat IgG, human IgG3 and mouse IgG1.In addition, MabAffinity Protein G Agarose High Flow can in some cases be used for Fab and F(ab)2 purification because of its binding affinity to CH1 domain of certain Fab.
  1. Binding specificities that complement Protein A media

  2. Suitable for different applications, including poly or monoclonal antibodies of different species and subclasses for its broad binding specificity

  3. No albumin binding domain and avoid undesirable interactions with albumin

  4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

CompositionHighly cross-linked Agarose
Average particle size90 micron
Form5ml prepacked column
LigandProtein G
Coupling chemistryEpoxy
Dynamic binding capacity1> 90mg IgG /5ml prepacked column
Recommended flow rate1-5ml/min for 5ml prepacked column
Column dimension1.6x2.5 cm for 5ml prepacked column
Maximum Pressure Drop0.3Mpa
Chemical stabilityStable in all aqueous buffers commonly used in protein G chromatography
pH working range2-9
pH CIP range (short term)2-10
CIP stability8M Urea, 6M Gua-HCl, 0.1M acetic acid
Temperature stability12-40oC
Storage20% Ethanol
Shelf life5 years
  1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

MabAffinity Protein G Agarose High Flow Resin belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity Protein G Agarose High Flow are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

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Price(USD) : $350.00

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