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Your Position: Home > IgG kits > Spike RBD > RAS-T136

Monkey Anti-SARS-CoV-2 (BA.5) Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
IDComponentsSize
RAS136-C01Pre-coated SARS-CoV-2 Spike RBD (BA.5) Microplate1 plate
RAS136-C02Positive Control100 μL
RAS136-C03Negative Control100 μL
RAS136-C04HRP-Goat anti-Monkey IgG50 μL
RAS136-C0510xWashing Buffer50 mL
RAS136-C06Dilution Buffer50 mL
RAS136-C07Substrate Solution12 mL
RAS136-C08Stop Solution7 mL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
  • Application
    The kit is developed for titer measurement of Anti-SARS-CoV-2 (BA.5) IgG antibody (Spike RBD) in Monkey serum.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike RBD(BA.5). The kit consists of Pre-coated SARS-CoV-2 Spike RBD (BA.5) Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti monkey  IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey  IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data  Please refer to DS document for The assay protocol.
 Spike RBD ELISA

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

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Price(USD) : $650.00

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