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Your Position: Home > > Anti-CD28 Antibody > CRS-A014

Anti-CD28 Antibody ELISA Kit

For research use only.
IDComponentsSize
CRS014-C01Pre-coated Human CD28 Microplate1 plate
CRS014-C02Anti-CD28 Antibody Standard20 μg
CRS014-C03HRP-Goat anti-Mouse IgG 10 μg
CRS014-C0410xWashing Buffer 50 mL
CRS014-C052xDilution Buffer50 mL
CRS014-C06Substrate Solution12 mL
CRS014-C07Stop Solution7 mL
  • Background
    Similar to CD3 antibody, CD28 antibodies also can be used to stimulate the proliferation and activation of T cells in CIK cell therapy. Under the cooperation of other cytokines, such as IL2 and IL1a, CIK cells with rapid proliferation, high tumoricidal activity, broad tumor killing spectrum and non-MHC-restricted tumor killing characteristics are generated, which has significant effects on the treatment of cancer, chronic leukemia, liver disease and neurological diseases. Obviously, It is necessary to control the residues of raw materials in the final cell therapy products.
  • Application
    The kit is developed for the detection of anti-CD28 antibody in Bioprocess manufacturing applications. It is used as a tool to aid in routine quality control of in-process streams as well as final product.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-CD28 antibodies. The kit consists of Pre-coated Human CD28 Microplate and Anti-CD28 Antibody Standard and HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the Anti-CD28 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add a diluted HRP-Goat anti-Mouse IgG to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.

Typical Data Please refer to DS document for The assay protocol.
 Anti-CD28 Antibody ELISA

Detection of Monoclonal Anti-CD28 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD28 can bind Anti-CD28 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.16 ng/mL (QC tested).

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