|CRS015-C01||Pre-coated Human CD3E & CD3G Microplate||1 plate|
|CRS015-C02||Anti-CD3 Antibody Standard||50 μL|
|CRS015-C03||HRP-Goat anti-Mouse IgG||10 μg|
|CRS015-C04||10xWashing Buffer||50 mL|
|CRS015-C05||2xDilution Buffer||50 mL|
|CRS015-C06||Substrate Solution||12 mL|
|CRS015-C07||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 31 days from the date of opening.
Your experiment will include 5 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Add your sample to the plate, take the Anti-CD3 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted HRP-Goat anti-Mouse IgG to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.
Detection of Monoclonal Anti-CD3 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD3E & CD3G can bind Anti-CD3 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.098 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $600.00
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