This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 53.0 kDa.
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
5e5 of anti-EGFRvIII CAR-293 cells were stained with 100 μL of 20 μg/mL of Alexa Fluor 488-Labeled Human EGFRvIII, His Tag (Cat. No. EGI-HA2H7) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
5e5 of PBMCs were stained with Alexa Fluor 488-Labeled Human EGFRvIII, His Tag (Cat. No. EGI-HA2H7) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $420.00
Price(USD) : $2616.00
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