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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 16.3 kDa.
>90% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Alpha-Synuclein monomer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Thioflavin T is a sensitive fluorescence reporter of fibrils formulation that binds to beta sheet-rich structures. The alpha synuclein pre-formed fibrils (Cat. No. ALN-H51H4) is able to induce the aggregation of alpha synuclein monomers (Cat. No. ALN-H52H8) (QC tested).
Transmission electron microscopy (TEM) of alpha-synuclein preformed fibrils (Cat. No. ALN-H51H4). Fibril structure is visible on negative stain TEM images of ALN-H51H4 (Routinely tested).
HEK293/Human Alpha-Synuclein (GFP) Stable Cell Line (Cat. No. CHEK-ATP085) were transduced with Human Alpha-Synuclein Pre-formed Fibrils, His Tag (Cat. No. ALN-H51H4) and Human Alpha-Synuclein, His Tag (Cat. No. ALN-H52H8) respectively. The fluorescence of GFP-Alpha-Synuclein (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Alpha-Synuclein, His Tag transduction. C. Lipo2000 and Human Alpha-Synuclein Pre-formed Fibrils, His Tag transduction. Scale bars, 50 μm (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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ACROBiosystems offers a wide selection of Matrigengel basement membrane extracts. Different versions – including phenol red-free, high concentration, iPSC culturing, organoid culturing – are all available for your specific needs to support 3D cell culture!
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