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Your Position: Home > Cytokines > LIF > ActiveMax® Human LIF Protein, Tag Free

ActiveMax® Human LIF Protein, Tag Free

  • Synonym
    LIF,CDF,DIA,HILDA,MLPLI
  • Source
    ActiveMax® Human LIF, Tag Free (LIF-H521b) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1).
    Predicted N-terminus: Ser 23
  • Molecular Characterization
    Online(Ser 23 - Phe 202) AAH69540.1

    This protein carries no "tag".

    The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human LIF, Tag Free (Cat. No. LIF-H521b) SDS-PAGE gel

ActiveMax® Human LIF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
ActiveMax® Human LIF, Tag FreeActiveMax® Human LIF, Tag Free (Cat. No. LIF-H521b) ELISA bioactivity

Immobilized ActiveMax® Human LIF, Tag Free (Cat. No. LIF-H521b) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 0.016-0.25 μg/mL (QC tested).

Please do not use together with anti His secondary antibodyin order to avoid high background
  • Citations
  • Background
    Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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