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Your Position: Home > Protein > Hemagglutinin 1 (HA1) > HA1-V82E3

Biotinylated Influenza A [A/Hong Kong/483/97(H5N1)] Hemagglutinin 1 (HA1) Protein, His Tag,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • Synonym
    HA1,Hemagglutinin 1
  • Source
    Biotinylated Influenza A [A/Hong Kong/483/97(H5N1)] HA1 Protein, His Tag,Avitag(HA1-V82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 17 - Arg 339 (Accession # O92930_9INFA ).
    Predicted N-terminus: Asp 17
  • Molecular Characterization
    Hemagglutinin 1 (HA1) Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 43.4 kDa. The protein migrates as 55-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Hemagglutinin 1 (HA1) SDS-PAGE

Biotinylated Influenza A [A/Hong Kong/483/97(H5N1)] HA1 Protein, His Tag,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Hemagglutinin 1 (HA1) MALS images

The purity of Biotinylated Influenza A [A/Hong Kong/483/97(H5N1)] HA1 Protein, His Tag,Avitag (Cat. No. HA1-V82E3) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Hemagglutinin 1 (HA1) ELISA

Immobilized Biotinylated Influenza A [A/Hong Kong/483/97(H5N1)] Hemagglutinin 1 (HA1) Protein, His Tag,Avitag (Cat. No. HA1-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Influenza A H5N1 Hemagglutinin Protein (HA1 Subunit) Antibody, Mouse IgG1 (QC tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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