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Your Position: Home > Immune Checkpoint Proteins > CD47 > Human CD47 Protein, Fc Tag (HPLC-verified)

Human CD47 Protein, Fc Tag (HPLC-verified)

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Human CD47, Fc Tag (HPLC-verified) (CD7-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Pro 139) NP_942088

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) SDS-PAGE gel

Human CD47, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human CD47, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human CD47, Fc Tag (HPLC-verified)Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) ELISA bioactivity

Immobilized Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) with a linear range of 16-250 ng/mL (QC tested).

Human CD47, Fc Tag (HPLC-verified)Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) ELISA bioactivity

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256): Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Immobilized Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=15.02 μg/mL (Routinely tested).

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