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Your Position: Home > Bio-Markers & CD Antigens > E-Cadherin > Human E‑Cadherin ECAD Protein, With C-Fc Tag

Human E-Cadherin / Cadherin-1 Protein, Fc Tag

  • Synonym
    Cadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO
  • Source
    Human E-Cadherin, Fc Tag (ECD-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 621 (Accession # AAI41839).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    E-Cadherin(Gln 23 - Pro 621) AAI41839

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 92.4 kDa. As a result of different glycosylation, the protein migrates as 110-125 kDa Cadherin, a 48kDa Propeptide under reducing (R) condition, and 200-250 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human E-Cadherin, Fc Tag (Cat. No. ECD-H5250) SDS-PAGE gel

The purity of rhECAD Fc Chimera was determined by SDS-PAGE of reduced (R) and non-reduced (NR) rhECAD Fc Chimera and staining overnight with Coomassie Blue.

Bioactivity
Human E-Cadherin, Fc TagHuman E-Cadherin, Fc Tag (Cat. No. ECD-H5250) ELISA bioactivity

Immobilized Human ITGAE&ITGB7, His Tag (Cat. No. IT7-H52W7) at 5 μg/mL (100 μL/well) can bind Human E-Cadherin, Fc Tag (Cat. No. ECD-H5250) with a linear range of 0.08-2.5 μg/mL (QC tested).

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Price(USD) : $260.00

Price(USD) : $1600.00

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