>All Other Proteins
>Human E‑Cadherin ECAD Protein, With C-Fc Tag
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 92.4 kDa. As a result of different glycosylation, the protein migrates as 110-125 kDa Cadherin, a 48kDa Propeptide under reducing (R) condition, and 200-250 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
The purity of rhECAD Fc Chimera was determined by SDS-PAGE of reduced (R) and non-reduced (NR) rhECAD Fc Chimera and staining overnight with Coomassie Blue.
Authors: Kevin Suffoletto, et al.
Journal: J Biomech 2018
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