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Your Position: Home > Protein > E-Cadherin > ECD-H5250

Human E-Cadherin / Cadherin-1 Protein, Fc Tag (SPR verified)

  • Synonym
    Cadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO
  • Source
    Human E-Cadherin, Fc Tag(ECD-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 621 (Accession # AAI41839 ).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    E-Cadherin Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 92.3 kDa. The protein migrates as 100 kDa and 120 kDa (Cadherin), and 48 kDa (Propeptide) under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
E-Cadherin SDS-PAGE

Human E-Cadherin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
 E-Cadherin SPR

Mouse KLRG1, His Tag (Cat. No. KL1-M5249) immobilized on CM5 Chip can bind Human E-Cadherin, Fc Tag (Cat. No. ECD-H5250) with an affinity constant of 0.699 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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