Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) is commmanded for DNA extration.
The kit is used for quantitative detection of residual CHO DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the CHO resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 6 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of CHO genomic DNA (included in the kit).
Figure 3. Consistent quantitation across a broad range of fragment sizes. Standard curves were generated using a 10-fold serial dilution of gDNA and fragmented DNA. Fragmented DNA was generated by sonicating total CHO genomic DNA (10 min, 20 min, 30min).
Figure 4. The amplification efficiency of standard curve (ACRO and Competitor J) is close to 100%, while the amplification efficiency of standard curve (Competitor H) is less than 90%.
Figure 5. The validation of spike recovery in three spike levels CHO DNA samples (high, medium, low). Results show that the recovery using the ACRO products are between 90 and 100%.
|OPA-R004-01||2×qPCR Master Mix||1.0 mL×2|
|OPA-R004-02||CHO Primer & Probe Mix||700 μL|
|OPA-R004-03||CHO DNA Control（3ng/μL）||100 μL|
|OPA-R004-04||Dilution Buffer||1.5 mL×2|
|OPA-R004-05||DNase/RNase-Free Water||1.0 mL|
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $1039.00
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