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Your Position: Home > Immune Checkpoint Proteins > CD27 > Human CD27 /TNFRSF7 Protein, With C-Fc Tag

Human CD27 / TNFRSF7 Protein, Fc Tag

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Human CD27, Fc Tag (CD7-H5254) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # NP_001233).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    Online(Thr 21 - Ile 192) NP_001233

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 45.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD27, Fc Tag (Cat. No. CD7-H5254) SDS-PAGE gel

Human CD27, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD27, Fc TagHuman CD27, Fc Tag (Cat. No. CD7-H5254) ELISA bioactivity

Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) with a linear range of 1-31 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Flow Cytometry assay shows that Human CD27, Fc Tag (Cat. No. CD7-H5254) can bind to Raji cells Surface Human CD70. The concentration of CD27 used is 1 μg/mL (Routinely tested).

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