Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) is commmanded for DNA extration.
The kit is used for quantitative detection of residual HEK293 DNA in biopharmaceutical production.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the HEK293 resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 5 (30 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of HEK293 genomic DNA (included in the kit) in the presence of CHO DNA and E. coli DNA.
|OPA-R006-01||2×qPCR Master Mix||1.0 mL×2|
|OPA-R006-02||HEK293 Primer & Probe Mix||700 μL|
|OPA-R006-03||HEK293 DNA Control (3 ng/μL)||100 μL|
|OPA-R006-04||Dilution Buffer||1.5 mL×2|
|OPA-R006-05||DNase/RNase-Free Water||1.0 mL|
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $1539.00
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