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Your Position: Home > Isotype Controls > IgG4 H Chain > Recombinant Human IgG H Chain Protein

Human IgG4 H Chain C Region (Full Length) Protein, Tag Free

  • Synonym
    IGHG4
  • Source
    Human IgG4 H Chain C Region (Full Length), Tag Free (IG4-H5218) is expressed from human 293 cells (HEK293). It contains AA Ala 1 - Lys 327 (Accession # P01861).
    Predicted N-terminus: Ala 1
  • Molecular Characterization
    IgG4 H Chain(Ala 1 - Lys 327) P01861

    This protein carries no "tag".

    The protein has a calculated MW of 35.9 kDa. The protein migrates as 35 kDa and 43-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human IgG4 H Chain C Region (Full Length), Tag Free (Cat. No. IG4-H5218) SDS-PAGE gel

Human IgG4 H Chain C Region (Full Length), Tag Free on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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