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Your Position: Home > Immune Checkpoint Proteins > Human ICOSLG /B7-H2 /CD275 Protein

Human B7-H2 / ICOSLG Protein

  • Synonym
    ICOSLG,B7-H2,B7H2,B7RP-1,B7RP1,CD275,GL50,ICOS-L,ICOSL,LICOS,ICOS ligand
  • Source
    Human B7-H2, His Tag (B72-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Ser 258 (Accession # NP_056074).
    Predicted N-terminus: Asp 19
  • Molecular Characterization
    B7-H2(Asp 19 - Ser 258) NP_056074

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.5 kDa. The protein migrates as 35-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H2, His Tag (Cat. No. B72-H5221) SDS-PAGE gel

Human B7-H2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human B7-H2, His TagHuman B7-H2, His Tag (Cat. No. B72-H5221) ELISA bioactivity

Immobilized Human B7-H2, His Tag (Cat. No. B72-H5221) at 1 μg/mL (100 µl/well),can bind Human ICOS, Fc Tag (Cat. No. ICS-H5250) with a linear of 0.07-2.3 ng/mL (QC tested).

  • Background
    ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $270.00

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Price(USD) : $1200.00

Inventory : In stock

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