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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Human CTLA4 /CD152 Protein, With C-Fc Tag

Human CTLA-4 / CD152 Protein, Fc Tag

  • Synonym
  • Source
    Human CTLA-4, Fc Tag (CT4-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 37 -  Ser 160 (Accession # NP_005205.2).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    CTLA-4(Ala 37 -  Ser 160) NP_005205.2

    This protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Ala 37. As a result of glycosylation, the reducing (R) protein migrates as 50-55 kDa, and the non-reducing (NR) protein migrates as 100-110 kDa in SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 35 mM Tris, 50 mM NaCl, 50 mM Glycine, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) SDS-PAGE gel

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 10 μg/mL (100 μL/well) can bind Human B7-1, His Tag (Cat. No. B71-H5228) with a linear range of 0.156-1.25 μg/mL (Routinely tested).

Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Immobilized Human B7-2, His Tag (Cat. No. CD6-H5223) at 10 μg/mL (100 µl/well), can bind Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) with a linear range of 0.04-0.3 μg/mL (QC tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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