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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Human CTLA4 /CD152 Protein, With C-Fc Tag

Human CTLA-4 / CD152 Protein, Fc Tag

  • Synonym
    CTLA4,CD152
  • Source
    Human CTLA-4, Fc Tag (CT4-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 37 -  Ser 160 (Accession # NP_005205.2).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    Online(Ala 37 -  Ser 160) NP_005205.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.0 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) SDS-PAGE gel

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA
Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Immobilized Human B7-2, His Tag (Cat. No. CD6-H5223) at 10 μg/mL (100 μL/well) can bind Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) with a linear range of 2-39 ng/mL (QC tested).

Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 10 μg/mL (100 μL/well) can bind Human B7-1, His Tag (Cat. No. B71-H5228) with a linear range of 0.156-1.25 μg/mL (Routinely tested).

Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

Human CTLA-4, Fc TagHuman CTLA-4, Fc Tag (Cat. No. CT4-H5255) ELISA bioactivity

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-2, Fc,Avitag (Cat. No. CD6-H82F5) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 μg/mL (Routinely tested).

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