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Your Position: Home > MABSol® Biotin Labeled Proteins > Growth Hormone R > Human GHR /GHBP Protein, Biotinylated

Biotinylated Human Growth Hormone R, ultra sensitivity (primary amine labeling)

  • Synonym
    GHR,GHBP,GH receptor
  • Source
    MABSol® Biotinylated Human Growth Hormone R (GHR) (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912). It is the biotinylated form of Human Growth Hormone R (GHR) Protein (Cat # GHR-H5222).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    GHR(Ala 27 - Tyr 264) P10912

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1-3 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Growth Hormone R (Cat. No. GHR-H8222) SDS-PAGE gel

Biotinylated Human Growth Hormone R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human Growth Hormone RBiotinylated Human Growth Hormone R (Cat. No. GHR-H8222) ELISA bioactivity

Immobilized Human GH, Tag Free (Cat. No. GH1-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GHR, His Tag (Cat. No. GHR-H8222) with a linear range of 2-31 ng/mL (QC tested).

  • Background
    Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

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