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Your Position: Home > Isotype Controls > IgG2a Fc > IGA-M5207

Mouse IgG2a Fc Protein, Tag Free

  • Synonym
    IgG2A
  • Source
    Mouse IgG2a Fc, Tag Free (IGA-M5207) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # CAC20702).
    Predicted N-terminus: Glu 98
  • Molecular Characterization
    Online(Glu 98 - Lys 330) CAC20702

    This protein carries no "tag".

    The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 30-35 kDa under reducing (R) condition, and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse IgG2a Fc, Tag Free (Cat. No. IGA-M5207) SDS-PAGE gel

Mouse IgG2a Fc, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified) (Cat. No. FCM-M52W2) on NTA Biosensor, can bind Mouse IgG2a Fc, Tag Free(Cat. No. IGA-M5207) with an affinity constant of 7.73 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse FCGRT&B2M Heterodimer Protein, His Tag (BLI verified) (Cat. No. FCM-M52W8) on NTA Biosensor, can bind Mouse IgG2a Fc, Tag Free(Cat. No. IGA-M5207) with an affinity constant of 5.75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $260.00

Price(USD) : $600.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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