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Your Position: Home > Bio-Markers & CD Antigens > Cystatin SN > Human CST1 /Cystatin-SN Protein

Human Cystatin SN / CST1 Protein

  • Synonym
    CST1,Cystatin-SN,Cystain-SA-I,Cystatin-1
  • Source
    Human Cystatin SN, His Tag (CS1-H5225) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Ser 141 (Accession # AAH21225).
    Predicted N-terminus: Trp 21
  • Molecular Characterization
    Cystatin SN(Trp 21 - Ser 141) AAH21225

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.1 kDa. The protein migrates as 17-19 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Cystatin SN, His Tag (Cat. No. CS1-H5225) SDS-PAGE gel

Human Cystatin SN, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Cystatin-1 (CST1) is also known as Cystatin-SN, Cystain-SA-I, Salivary cystatin-SA-1, which is secreted protein and belongs to the cystatin family. Cystatin-1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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