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Your Position: Home > Receptor Tyrosine Kinases > Ephrin-B1 > Human Ephrin-B1 /EFNB1 Protein

Human Ephrin-B1 / EFNB1 Protein

  • Synonym
    EFNB1,EFL-3,ELK ligand,ELK-L,EPLG2,LERK2,CFND,CFNS
  • Source
    Human Ephrin-B1, His Tag (EF1-H5223) is expressed from human 293 cells (HEK293). It contains AA Leu 28 - Gly 232 (Accession # NP_004420).
    Predicted N-terminus: Leu 28
  • Molecular Characterization
    Ephrin-B1(Leu 28 - Gly 232) NP_004420

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.2 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Ephrin-B1, His Tag (Cat. No. EF1-H5223) SDS-PAGE gel

Human Ephrin-B1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Ephrin-B1 (EFNB1) is also known as EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2 (LERK-2), which belongs to the ephrin family. EFNB1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNB1 binds to the receptor tyrosine kinases EPHB1 and EPHA1. EFNB1 binds to, and induce the collapse of, commissural axons/growth cones in vitro. EFNB1 may play a role in constraining the orientation of longitudinally projecting axons. EFNB1 interacts with GRIP1 and GRIP2.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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