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Your Position: Home > All Other Proteins > Activin RIB > Human ACVR1B /ALK4 Protein, With C-Fc Tag

Human Activin RIB / ACVR1B Protein, Fc Tag

  • Synonym
    ACVR1b,ALK4,ACVRLK4,ACTR-IB,SKR2
  • Source
    Human Activin RIB, Fc Tag (ACB-H5251) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Glu 126 (Accession # AAH00254).
    Predicted N-terminus: Ser 24
  • Molecular Characterization
    ACVR1B(Ser 24 - Glu 126) AAH00254

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 38 kDa. As a result of glycosylation, the protein migrates as 43-46 kDa under reducing (R) condition, and 85-90 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Activin RIB, Fc Tag (Cat. No. ACB-H5251) SDS-PAGE gel

Human Activin RIB, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Human Activin RIB, Fc TagHuman Activin RIB, Fc Tag (Cat. No. ACB-H5251) ELISA bioactivity

Immobilized Human Activin A, Tag Free (Cat. No. ACA-H421b) at 10μg/mL (100 μL/well) can bind Human ACVR1B, Fc Tag (Cat. No. ACB-H5251) with a linear range of 0.3-2.5 μg/mL (QC tested).

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