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Your Position: Home > All Other Proteins > Osteoprotegerin > Human TNFRSF11B /Osteoprotegerin Protein,C-Fc Tag,HEK293 expressed

Human Osteoprotegerin / TNFRSF11B Protein, Fc Tag

  • Synonym
    TNFRSF11B,OCIF,OPG,Osteoprotegerin
  • Source
    Human OPG, Fc Tag (TNB-H5259) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537).
    Predicted N-terminus: Glu 22
  • Molecular Characterization
    Online(Glu 22 -  Leu 401) NP_002537

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 70.2 kDa. As a result of glycosylation, the protein migrates as 66-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human OPG, Fc Tag (Cat. No. TNB-H5259) SDS-PAGE gel

Human OPG, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Human OPG, Fc TagHuman OPG, Fc Tag (Cat. No. TNB-H5259) ELISA bioactivity

Immobilized Human TNFSF11, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human OPG, Fc Tag (Cat. No. TNB-H5259) with a linear range of 0.6-5 ng/mL (QC tested).

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