Human FGF R1 / CD331 Protein, Strep Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
FGFR1,BFGFR,CD331,CEK,FGFBR,FLG,HBGFR,N-SAM,FLT2,H2,KAL2,FLJ14326
Source
Human FGF R1, Strep Tag (FG1-H5289) is expressed from human 293 cells (HEK293). It contains AA Arg 22 - Ile 376 (Accession # AAH18128).
Predicted N-terminus: Arg 22
Request for sequence
Molecular Characterization
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 42.2 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human FGF R1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Background
Fibroblast growth factor receptor 1 (FGFR1) is also known as basic fibroblast growth factor receptor 1(BFGFR1), FMS-like tyrosine kinase, CD331, and is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. This protein is one of several fibroblast growth factor receptors, which are related proteins that are involved in important processes such as cell division, regulation of cell growth and maturation, formation of blood vessels, wound healing, and embryonic development.The FGFR1 protein spans the cell membrane, so that one end of the protein remains inside the cell and the other end projects from the outer surface of the cell. This positioning allows the FGFR1 protein to interact with specific growth factors outside the cell and to receive signals that help the cell respond to its environment. When growth factors attach to the FGFR1 protein, the receptor triggers a cascade of chemical reactions inside the cell that instruct the cell to undergo certain changes, such as maturing to take on specialized functions. The FGFR1 protein is thought to play an important role in the development of the nervous system. This protein may also help regulate the growth of long bones, such as the large bones in the arms and legs.
References
- (1) Elbauomy Elsheikh S,et al., 2007, Breast Cancer Res.,9(2):R23.
- (2) Macdonald D,et al., 2002, Acta Haematol. 107(2):101-7.
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