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Your Position: Home > All Other Proteins > LDL R > LDR-H5281

Human LDL R (High Purity) Protein, Strep Tag

  • Synonym
    LDLR,FH,FHC,LDLCQ2
  • Source
    Human LDL R, Strep Tag (LDR-H5281) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Arg 788 (Accession # NP_000518.1).
    Predicted N-terminus: Ala 22
  • Molecular Characterization
    Online(Ala 22 - Arg 788) NP_000518.1

    This protein carries a twin strep tag at the C-terminus.

    The protein has a calculated MW of 87.8 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LDL R, Strep Tag (Cat. No. LDR-H5281) SDS-PAGE gel

Human LDL R, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human LDL R, Strep TagHuman LDL R, Strep Tag (Cat. No. LDR-H5281) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 2 μg/mL (100 μL/well) can bind Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) with a linear range of 1-15.6 ng/mL (QC tested).

  • Background
    Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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