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Your Position: Home > Antibody > Hemagglutinin (HA) > HA2-Y290

Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5)

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  • Source

    Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    13A5

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Specificity

    This product is a specific antibody specifically reacts with HA.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-50 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Cross Verification

    This product can cross in Elisa with
    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
    Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
    Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
    This product No cross-reactivity in ELISA with Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
    Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
    Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
    Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4).
    Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
    Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
    Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
    Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
    Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
    Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
    Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
    Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
    Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
    Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).

SDS-PAGE
Hemagglutinin (HA) SDS-PAGE

Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Hemagglutinin (HA) ELISA

Immobilized Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (QC tested).

 Hemagglutinin (HA) ELISA

Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.2-13 ng/mL (Routinely tested).

 Hemagglutinin (HA) ELISA

Immobilized Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (Routinely tested).

Bioactivity-SPR
 Hemagglutinin (HA) SPR

Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with an affinity constant of 3.43 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Clinical and Translational Updates

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Price(USD) : $350.00

Price(USD) : $2310.00

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