This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.0 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human Mesothelin (aa 296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human Mesothelin (aa 296-580), His Tag (Cat. No. MSN-H5223) with an affinity constant of 0.52 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin / MSLN (296-580) Protein, His Tag (Cat. No. MSN-H5223, 1µg/ml) and C. His Tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His Tag-protein control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin / MSLN (296-580) Protein, His Tag (Cat. No. MSN-H5223).
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