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Your Position: Home > All Other Proteins > Mesothelin > Human MSLN /Mesothelin (aa 296-580) Protein

Human Mesothelin / MSLN (296-580) Protein

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Human Mesothelin, His Tag (MSN-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Online(Glu 296 - Gly 580) AAH09272

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.0 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Mesothelin, His Tag (Cat. No. MSN-H5223) SDS-PAGE gel

Human Mesothelin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Mesothelin, His TagHuman Mesothelin, His Tag (Cat. No. MSN-H5223) ELISA bioactivity

Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-2 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human Mesothelin (aa 296-580), His Tag (Cat. No. MSN-H5223) with an affinity constant of 0.52 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin / MSLN (296-580) Protein, His Tag (Cat. No. MSN-H5223, 1µg/ml) and C. His Tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His Tag-protein control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin / MSLN (296-580) Protein, His Tag (Cat. No. MSN-H5223).

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Price(USD) : $1980.00

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