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Your Position: Home > All Other Proteins > Mesothelin > Human MSLN /Mesothelin (aa 296-580) Protein, With N-Fc Tag

Human Mesothelin / MSLN Protein, Fc Tag

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Human Mesothelin (aa 296-580), Fc Tag (MSN-H526x) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Mesothelin(Glu 296 - Gly 580) AAH09272

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 59.6 kDa. As a result of glycosylation, the protein migrates as 60-68 kDa under reducing (R) condition, and 120-130 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Mesothelin (aa 296-580), Fc Tag (Cat. No. MSN-H526x) SDS-PAGE gel

Human Mesothelin (aa 296-580), Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Mesothelin (aa 296-580), Fc TagHuman Mesothelin (aa 296-580), Fc Tag (Cat. No. MSN-H526x) ELISA bioactivity

Immobilized Human Mesothelin, Fc Tag (Cat. No. MSN-H526x) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.8-3 ng/mL (QC tested).

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