Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antibody > Glycoprotein E2 & E1 > GL2-MY2095

Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (MALS verified)

Order Now

  • Source

    Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    4D1

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV) is expressed from Baculovirus-Insect cells.

  • Specificity

    Specifically recognizes Rubella virus Glycoprotein E1 (strain M33) (RUBV).

  • Application
    ApplicationRecommended Usage
    Western Blot10 ug/mL
    ELISA0.3-78 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein E2 & E1 SDS-PAGE

Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Glycoprotein E2 & E1 MALS images

The purity of Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (Cat. No. GL2-MY2095) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glycoprotein E2 & E1 ELISA

Immobilized Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag (Cat. No. GL2-R5583) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (Cat. No. GL2-MY2095) with a linear range of 0.3-5 ng/mL (QC tested).

Western Blot
 Glycoprotein E2 & E1 WESTERN BLOT

Detection of Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa ,HEK by Western Blot. Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa, HEK at 10 ug/ml dilution + Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag (MALS verified), His Tag at 400ng.

Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.

Predicted band size: 95 kDa 12% Bis-Tris Protein Gel.

  • Background
    Rubella virus (RV), the etiological agent of German measles, is a small enveloped RNA virus that belongs to the togavirus family. RV virions contain two glycosylated membrane proteins, E1 and E2, that exist as a heterodimer and form the viral spike complexes on the virion surface. Formation of an E1-E2 heterodimer is required for transport of E1 out of the endoplasmic reticulum lumen to the Golgi apparatus and plasma membrane.
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $2310.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message