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Your Position: Home > All Other Proteins > DcR3 > Human TNFRSF6B /DcR3 Protein,C-Fc Tag,HEK293 expressed

Human DcR3 / TNFRSF6B Protein, Fc Tag

  • Synonym
    TNFRSF6B,DCR3,TR6,M68
  • Source
    Human DcR3, Fc Tag (TNB-H5255) is expressed from human 293 cells (HEK293). It contains AA Val 30 - His 300 (Accession # O95407-1).
    Predicted N-terminus: Val 30
  • Molecular Characterization
    Online(Val 30 - His 300) O95407-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 56.2 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human DcR3, Fc Tag (Cat. No. TNB-H5255) SDS-PAGE gel

Human DcR3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human DcR3, Fc TagHuman DcR3, Fc Tag (Cat. No. TNB-H5255) ELISA bioactivity

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 5 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 5-20 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human DcR3, Fc Tag(Cat. No. TNB-H5255)on AHC Biosensor, can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin(Cat. No. LIT-H5256)with an affinity constant of 1.64 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human DcR3, Fc Tag (Cat. No. TNB-H5255) on AHC Biosensor, can bind Human LIGHT, His Tag (Cat. No. LIT-H5247) with an affinity constant of 4.94 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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