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Your Position: Home > Protein > Ubiquitin > UBN-H5144

Human Ubiquitin Protein, His Tag

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  • Synonym
    HEL-S-50
  • Source
    Human Ubiquitin Protein, His Tag(UBN-H5144) is expressed from E. coli cells. It contains AA Gln 2 - Gly 76 (Accession # P0CG47-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Ubiquitin Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 10.5 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Background
    Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein.
  • Clinical and Translational Updates

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