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Your Position: Home > Bio-Markers & CD Antigens > Human-Mature-PCSK9-(153-692)-Protein

Human Mature PCSK9 (153-692) Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Human Mature PCSK9 (153-692) (PC9-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 153 - Gln 692 (Accession # AAI66619).
    Predicted N-terminus: Ser 153
  • Molecular Characterization
    PCSK9(Ser 153 - Gln 692) AAI66619

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 58 kDa. The protein migrates as 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 10 mM HCl, pH2.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Mature PCSK9 (153-692) (Cat. No. PC9-H5226) SDS-PAGE gel

Human Mature PCSK9 (153-692) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human Mature PCSK9 (153-692)Human Mature PCSK9 (153-692) (Cat. No. PC9-H5226) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Human Mature PCSK9 (153-692) (Cat. No. PC9-H5226) with a linear range of 0.03-0.5 μg/mL (QC tested).

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