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Your Position: Home > All Other Proteins > BCMA > Human TNFRSF17 /CD269 /BCMA Protein, With C-Fc Tag

Human BCMA / TNFRSF17 Protein, Fc Tag

  • Synonym
  • Source
    Human BCMA, Fc Tag (BC7-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 1 -  Ala 54 (Accession # NP_001183.2).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Online(Met 1 -  Ala 54) NP_001183.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 32 kDa. As a result of glycosylation, the protein migrates as 35-40 kDa under reducing (R) condition, and 66 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Human BCMA, Fc Tag (Cat. No. BC7-H5254) SDS-PAGE gel

Human BCMA, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human BCMA, Fc TagHuman BCMA, Fc Tag (Cat. No. BC7-H5254) ELISA bioactivity

Immobilized Biotinylated Human BAFF, His Tag (Cat. No. BAF-H82Q2) at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with a linear range of 0.1-0.8 ng/mL (QC tested).

Human BCMA, Fc TagHuman BCMA, Fc Tag (Cat. No. BC7-H5254) ELISA bioactivity

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.1-1.6 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254, 3 μg/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254).

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