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Your Position: Home > All Other Proteins > BCMA > BC7-H5254

Human BCMA / TNFRSF17 Protein, Fc Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Human BCMA, Fc Tag (BC7-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # NP_001183.2).
    Predicted N-terminus: Met 1
    Request for sequence
  • Molecular Characterization
    Online(Met 1 - Ala 54) NP_001183.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 32.0 kDa. The protein migrates as 34-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BCMA, Fc Tag (Cat. No. BC7-H5254) SDS-PAGE gel

Human BCMA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human BCMA, Fc TagHuman BCMA, Fc Tag (Cat. No. BC7-H5254) ELISA bioactivity

Immobilized Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with a linear range of 0.1-0.8 ng/mL (QC tested).

Protocol
Human BCMA, Fc TagHuman BCMA, Fc Tag (Cat. No. BC7-H5254) ELISA bioactivity

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.1-1.6 ng/mL (Routinely tested).

Protocol
Human BCMA, Fc TagHuman BCMA, Fc Tag (Cat. No. BC7-H5254) ELISA bioactivity

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 5 μg/mL (100 μL/well) can bind Human APRIL, His Tag (Cat. No. APL-H5244) with a linear range of 8-125 ng/mL (Routinely tested).

Protocol
Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254, 3 μg/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254).

Protocol

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