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Your Position: Home > Protein > Polymerase acidic protein > PON-V51H3

Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag

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  • Source
    Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag(PON-V51H3) is expressed from E. coli cells. It contains AA Met 1 - Gly 209 (Accession # P31343-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Polymerase acidic protein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, 0.1% TritonX-100, 50% Glycerol, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Polymerase acidic protein SDS-PAGE

Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Polymerase acidic protein ELISA

Immobilized Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (Cat. No. PON-V51H3) at 1 μg/mL (100 μL/well) can bind Influenza A PA Polyclonal Antibody,Rabbit/IgG with a linear range of 0.2-16 ng/mL (QC tested).

  • Background
    The influenza virus RNA-dependent RNA polymerase is highly conserved among influenza A, B, C, and D viruses. It comprises three subunits: polymerase basic protein 1 (PB1), polymerase basic protein 2 (PB2), and polymerase acidic protein (PA) in influenza A and B viruses or polymerase 3 protein (P3) in influenza C and D viruses. Because this polymerase is essential for influenza virus replication, it has been considered as a target for antiviral agents.
  • Clinical and Translational Updates

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