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Your Position: Home > Kits > pTau181 > NTP-A03

Human Phospho Tau (Thr181) ELISA Kit

For research use only.

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  • Background
    Human Phospho Tau (Thr181) ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human pTau181 in cerebrospinal fluid, serum, plasma or other biological fluids. An antibody specific for pTau181 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the pTau181 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-pTau181 Antibody, which also binds the pTau181 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-pTau181 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-pTau181 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of pTau181 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of pTau181 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
  • Application

    The kit is developed for the detection and quantitative determination of pTau181 in human cerebrospinal fluid or peripheral blood and cell culture supernates.

    It is for research use only.

  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    • Background: pTau181
      Tau, the microtubule‐associated protein, forms insoluble filaments that accumulate as neurofibrillary tangles in Alzheimer's disease (AD) and related tauopathies. Under physiological conditions, tau regulates the assembly and maintenance of the structural stability of microtubules. In the diseased brain, however, tau becomes abnormally hyperphosphorylated, which ultimately causes the microtubules to disassemble, and the free tau molecules aggregate into paired helical filaments.
    • Clinical and Translational Updates

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