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Your Position: Home > Antibody > IgG2b > IGB-MY2176

Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (MALS verified)

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  • Source

    Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    P1F04

  • Species

    Rabbit

  • Isotype

    Rabbit IgG | Rabbit Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Specifically recognizes Mouse IgG2b Fc,no recrecognizes Mouse IgG1 Fc,Mouse IgG2a Fc and Mouse IgG3 Fc.

  • Immunogen

    Recombinant Mouse IgG2b Fc Protein is expressed from human 293T cells.

  • Specificity

    This product is a specific antibody specifically reacts with IgG2b.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-63 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Cross Verification

    This product No cross-reactivity in ELISA with
    Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208).
    Mouse IgG2a Fc, Tag Free (Cat. No. IGA-M5207).
    Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).
    Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3).
    Monoclonal Anti-Human Phospho-Tau (Thr217) Antibody, Mouse IgM (6G7C8) (Cat. No. PT7-Y2074).
    Human IgG Fc, Tag Free (Cat. No. FCC-H5214).
    Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206).
    Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200).
    Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205).
    Mouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201).
    Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).

SDS-PAGE
IgG2b SDS-PAGE

Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IgG2b MALS images

The purity of Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IgG2b ELISA

Immobilized Mouse IgG2b Fc, Tag Free (Cat. No. IGB-M5203) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b.
  • Clinical and Translational Updates

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