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Your Position: Home > Protein > CD1d & B2M > CDM-HP2H5

PE-Labeled Human CD1d&B2M Tetramer Protein (Peptide free)

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  • Source
    PE-Labeled Human CD1d&B2M Tetramer Protein(CDM-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M) (Accession # P15813 (CD1d) & P61769 (B2M)).
    Predicted N-terminus: Glu 20 & Ile 21
  • Molecular Characterization

    PE-Labeled Human CD1d&B2M Tetramer Protein is assembled by biotinylated monomer (CDM-H82E9) and PE-labeled streptavidin.

    Biotinylated Human CD1d&B2M Monomer Protein is produced by co-expression of CD1d and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 CD1d & B2M ELISA

Immobilized PE-Labeled Human CD1d&B2M Tetramer Protein (Cat. No. CDM-HP2H5) at 1 μg/mL (100 μL/well) can bind Human CD1d Antibody, Mouse IgG2B with a linear range of 0.2-16 ng/mL (QC tested).

 CD1d & B2M ELISA

Immobilized PE-Labeled Human CD1d&B2M Tetramer Protein (Cat. No. CDM-HP2H5) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).

  • Background
    CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mice. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d restricted NKT cells.
  • Clinical and Translational Updates

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Price(USD) : $410.00

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