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Your Position: Home > Protein > FASN > FAN-H5143

Human FASN Protein, His Tag (active enzyme)

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  • Synonym
    FAS, OA-519, SDR27X1
  • Source
    Human FASN Protein, His Tag(FAN-H5143) is expressed from E. coli cells. It contains AA Leu 2200 - Gly 2511 (Accession # NP_004095-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    FASN Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 58.3 kDa. The protein migrates as 34-39 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.9 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
FASN SDS-PAGE

Human FASN Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity

Measured by its ability to hydrolyze 4-methylumbelliferyl heptanoate. The specific activity is >3 pmol/min/μg (QC tested).

  • Background
    The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
  • Clinical and Translational Updates

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