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Your Position: Home > Protein > Laminin 511 > LA1-H5314

Human Laminin 511 Protein, premium grade (Mix & Scale)

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  • Product Details
    Human Laminin 511 Protein, premium grade (Mix & Scale) is a recombinant human laminin 511 E8 (LN511-E8) protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs).
    As published by Takamichi Miyazaki et al., the LN511-E8 variant of laminin 511 shows higher efficiency for supporting the adhesion of dissociated cells than did wild-type laminin 511 which makes it a cost-effective choice.
    LN511-E8 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC, etc. In addition, LN511-E8 has been demonstrated to support PSC growth for > 10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages.
    Importantly, our innovative laminin 511 protein (Mix & Scale) could meanwhile support pre-coating method and suspension method (directly mixing the protein with cell suspensions without coating plates).
    No coating steps are needed and easy scale-up
    Simplified operation and support automated manufacturing at a large scale.
    Cost-effective
    Half of the amount (0.25 μg/cm2) is used in the coating method and saves money.
    Stemness maintenance
    No spontaneous differentiation is observed after several passages of hPSC culture.
    Lot-to-lot consistency
    Produced from a stable cell line, robust purification process, and stringent QC.
    Ready to scale supply
    A new cGMP-compliant facility is ready and supports large scale manufacutring supply.
  • Key parameter
    Purity (SDS PAGE)> 95%
    Mycoplasma TestNegative
    Sterility TestNegative
    Endotoxin Test< 0.01 EU per μg
    Host Cell Protein< 0.5 ng/µg
    Host Cell DNA< 0.02 ng/μg
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -70°C.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 2-8°C for 6 months under sterile condition;
    2. -20°C or below for 2 years.
The schematic diagram of Laminin 511 (Mix & Scale) in ES/iPS cell culture.
Application Data
 Laminin 511 APPLICATION DATA

Robust, homologous, monolayer expansion of single cell hPSCs on Human Laminin 511 Protein, premium grade ((Mix &Scale) (Cat. No. LA1-H5314).

 Laminin 511 APPLICATION DATA

hPSCs cultured on Human Laminin 511 Protein, premium grade ((Mix &Scale) (Cat. No. LA1-H5314) maintain pluripotency, exhibiting high expression of OCT4, SOX2, and SSEA4 as confirmed by FACS and immunofluorescent staining.

Bioactivity-SPR
 Laminin 511 SPR

Human Laminin 511 Protein, premium grade (Mix & Scale) (Cat. No. LA1-H5314) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 5.73 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Clinical and Translational Updates

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