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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > Rat FcRN & B2M Heterodimer Protein

Rat FcRn/FCGRT & B2M Heterodimer Protein

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Rat FcRn, His Tag, Strep Tag (FCM-R5287) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_203502.1 (FCGRT) & NP_036644 (B2M)).
    Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_203502.1 (FCGRT) & NP_036644 (B2M)

    Rat FcRn, His Tag, Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 43-55 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat FcRn, His Tag, Strep Tag (Cat. No. FCM-R5287) SDS-PAGE gel

Rat FcRn, His Tag, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Rat FcRn, His Tag, Strep TagRat FcRn, His Tag, Strep Tag (Cat. No. FCM-R5287) ELISA bioactivity

Immobilized Ipilumimab at 5 μg/mL (100 μL/well) can bind Rat FcRn, His Tag, Strep Tag (Cat. No. FCM-R5287) with a linear range of 0.078-0.625 μg/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2400.00

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